[ Title ]
- RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics
[ Author ]
- Best, MG Sol, N Kooi, I Tannous, J Westerman, BA Rustenburg, F Schellen, P Verschueren, H Post, E Koster, J Ylstra, B Ameziane, N Dorsman, J Smit, EF Verheul, HM Noske, DP Reijneveld, JC Nilsson, RJA Tannous, BA Wesseling, P Wurdinger, T
[ Abstract ]
- Tumor-educated blood platelets (TEPs) are implicated as central players
in the systemic and local responses to tumor growth, thereby altering
their RNA profile. We determined the diagnostic potential of TEPs by
mRNA sequencing of 283 platelet samples. We distinguished 228 patients
with localized and metastasized tumors from 55 healthy individuals with
96% accuracy. Across six different tumor types, the location of the
primary tumor was correctly identified with 71% accuracy. Also, MET or
HER2-positive, and mutant KRAS, EGFR, or PIK3CA tumors were accurately
distinguished using surrogate TEP mRNA profiles. Our results indicate
that blood platelets provide a valuable platform for pan-cancer,
multiclass cancer, and companion diagnostics, possibly enabling clinical
advances in blood-based "liquid biopsies".
[ URL ]
- http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord;UT=WOS:000364609100014