[ Title ]
- Epithelial cancer biomarker EpCAM determination in peripheral blood
samples using a microfluidic immunosensor based in silver nanoparticles
as platform
[ Journal ]
- SENSORS AND ACTUATORS B-CHEMICAL
[ Author ]
- Ortega, FG
Fernandez-Baldo, MA
Serrano, MJ
Messina, GA
Lorente, JA
Raba, J
[ Abstract ]
- In the present work, we report a microfluidic immunosensor for
epithelial cancer biomarker EpCAM (epithelial cell adhesion molecule)
determination. It is based on the use of synthetized silver
nanoparticles (AgNPs) covered by chitosan (Cts). AgNPs-Cts were
covalently attached to the central channel (CC) of the microfluidic
immunosensor. These nanoparticles were employed as platform for
anti-EpCAM monoclonal antibodies immobilization for specifically
recognize and capture EpCAM in peripheral blood samples. Afterwards, the
amount of this trapped epithelial cancer biomarker was quantified by
HRP-conjugated anti-EpCAM-antibody. HRP reacted with its enzymatic
substrate in a redox process which resulted in the appearance of a
current whose magnitude was directly proportional to the level of EpCAM
in the peripheral blood sample. The structure and morphology of
synthetized AgNPs-Cts were characterized by UV-visible spectroscopy,
scanning electron microscopy (SEM), energy dispersive spectrometer (EDS)
and X-ray diffraction (XRD). The calculated detection limits for
microfluidic immunosensor and the commercial ELISA were 2.7 and 13.9 pg
mL(-1), respectively and the within- and between-assay coefficients of
variation were below 6.37% for the proposed method. The microfluidic
immunosensor is simple, sensitive, specific and reproducible. It has the
potential for reliable point-of-care clinical diagnosis and prognosis of
epithelial origin tumors in biological samples.
[ URL ]
- http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord;UT=WOS:000362918100033