[ Title ]
- Highly sensitive detection of cancer cells based on the DNA barcode assay and microcapillary electrophoretic analysis
[ Journal ]
- Electrophoresis
[ Author ]
- S. Chung, M. Cho, J. Hwan Jung and T. Seok Seo
[ Abstract ]
- Considering rarity of circulating tumor cells (CTCs) in human blood, the development of highly sensitive detection techniques for cancer cells is crucial for prediction, diagnosis, and prognosis of cancers. In this study, we propose an advanced cellular detection method by combining a biobarcode assay and microcapillary electrophoresis (CE) technology. While the DNA biobarcode assay can provide ultrasensitive and multiplex detection platforms, the CE chip can analyze barcode DNAs with high speed and accuracy according to the DNA size. We designed the barcode DNA size as 20 bp for indicating the expression of epithelial cell adhesion molecules (EpCAM) biomarkers and 30 bp for assigning CDX2 expression which is specific for colorectal cancer cells with addition to two bracket ladders (15 and 45 bp). Using MCF-7 (breast cancer) and SW620 (colorectal cancer) as models, we conducted a biobarcode assay and analyzed the resultant biobarcode DNA on the CE chip. We could detect the 20 bp CE peak in the electropherogram even with ten MCF-7 and SW620 cells in a volume of 200 L, thereby demonstrating the highly sensitive detection of cancer cells. We furthermore identified the type of colorectal cancer by observing two positive peaks (20 bp for EpCAM and 30 bp for CDX2) in the CE analysis.
[ URL ]
- ://WOS:000336500600015